Abstract:
Objective To explain the poor prognosis of new-onset diabetes complicating pancreatic cancer, the impact of insulin on the absorption of sodium by the pancreatic cancer cell (SW1990) was detected.
Methods The SW1990 cell line cultured in the high glucose DMEM medium was stimulated with insulin of gradient concentration, and the concentrations of Na+ in medium were detected at different time to explore the dose effect and time effect. The impact of insulin on serum and glucocorticoid-inducible kinase 1 (SGK1), and the possible signaling pathways were also studied.
Results The expression of SGK1 was remarkably increased(P<0.05) while the concentration of Na+ in cell culture solution decreased(P<0.05) after SW1990 was treated with insulin, and the descending range of Na+ was positively related to the SGK1 protein level(r=0.715, P=0.009); the expression of CREBBP and EP300 was closely associated with acetylation elevated apparently after insulin was added(P<0.05).
Conclusion Insulin can promote the absorption of sodium by the pancreatic cell line, which correlates to the transcription level of SGK1 gene, and acetylation may play a role in this process.
Key words:
Insulin,
Pancreatic cancer,
Diabetes mellitus,
Sodium,
SGK1,
Acetylation
Ya-dong SONG, Wen-chao GAO, Ru-fu CHEN, Xian-ying HE, Quan-bo ZHOU, Qing LIN, Yu ZHOU, Bing ZENG, Min YU, Zhi-hua LI. Mechanism of insulin promoting absorption of sodium by pancreatic cancer cell SW1990[J]. Chinese Archives of General Surgery(Electronic Edition), 2013, 07(06): 431-435.