Role of Oddi sphincter motility in the process of cholesterol gallbladder stone formation in guinea pigs

doi:10.3877/cma.j.issn.1674-0793.2015.05.005

Abstract

Abstract:

Objective

To investigate the role of Oddi sphincter motility in cholesterol gallbladder stone formation in model of guinea pigs.

Methods

Thirty-four adult male Hartley guinea pigs were divided randomly into two groups, control group (10 guinea pigs) and cholesterol stone groups (divided into 4 subgroups with 6 guinea pigs in each according to time they were sacrificed). The animals in cholesterol stone groups were fed with cholesterol lithogenic diet and sacrificed after 3, 6, 9 and 12 weeks. Sphincter of Oddi manometry and recording of myoelectric activity of the guinea pigs were obtained by multifunctional physiograph at each stage.

Results

The incidence of cholesterol gallstone formation was 0, 16.7%, 16.7% and 83.3% in 3 w, 6 w, 9 w and 12 w group. The frequency of myoelectric activity decreased apparently in the 3 w group and 6 w group (P<0.05). The amplitude of myoelectric activity decreased apparently, from (146.44±81.09) μV to (68.18±49.58) μV and (40.60±45.03) μV in the 9 w group and the 12 w group (P<0.05). The frequency of Oddi sphincter decreased apparently in the 6 w and 9 w group (P<0.05). The Oddi sphincter basal pressure and common bile duct pressure increased apparently in the 12 w group, from (25.19±7.77) mmHg to (52.38±12.84) mmHg and from (22.35±7.60) mmHg to (50.11±12.59) mmHg, respectively (P<0.01).

Conclusions

Cholesterol gallstone-causing diet can induce dysfunction of Oddi sphincter, the tension increased and its activity decreased. The disturbance of Oddi sphincter motility plays an important role in cholesterol gallstone formation.

Key words: Cholesterol gallstone, Oddi sphincter, Manometry, Myoelectric activity, Guinea pig

Chengsheng Gao, Chengkun Qin, Wenmao Geng, Xianping Cui, Guozhe Xian, Zhenhai Zhang. Role of Oddi sphincter motility in the process of cholesterol gallbladder stone formation in guinea pigs[J]. Chinese Archives of General Surgery(Electronic Edition), 2015, 09(05): 346-350.

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Figures/Tables 6

References 15

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组别	n	振幅	频率
对照组	10	146.44±81.09	15.86±4.35
3周组	6	100.27±50.42	10.89±2.65
6周组	6	152.96±81.05	8.68±2.35
9周组	6	68.18±49.58	13.70±2.88
12周组	6	40.60±45.03	13.80±3.79
t1, t2,t3,t4值	?	1.248, -0.156, 1.850, 2.913	2.513, 3.698, 1.075, 0.959
P1,P2,P3,P4值	?	>0.05, >0.05, <0.05, <0.05	<0.05, 0.05, >0.05

组别	n	振幅	频率
对照组	10	146.44±81.09	15.86±4.35
3周组	6	100.27±50.42	10.89±2.65
6周组	6	152.96±81.05	8.68±2.35
9周组	6	68.18±49.58	13.70±2.88
12周组	6	40.60±45.03	13.80±3.79
t1, t2,t3,t4值	?	1.248, -0.156, 1.850, 2.913	2.513, 3.698, 1.075, 0.959
P1,P2,P3,P4值	?	>0.05, >0.05, <0.05, <0.05	<0.05, 0.05, >0.05

组别	n	括约肌基础压力	胆总管压力	括约肌幅度	括约肌频率
对照组	10	25.19±7.77	22.35±7.60	8.52±2.27	11.57±2.94
3周组	6	21.25±5.38	18.48±4.94	8.33±3.85	11.50±1.64
6周组	6	27.57±8.67	25.82±8.26	5.26±2.40	7.67±3.44
9周组	6	34.11±11.56	32.15±11.64	5.89±1.41	7.33±3.27
12周组	6	52.38±12.84	50.11±12.59	5.11±2.59	6.60±3.51
t1, t2,t3,t4值	?	1.088, -0.569, -1.857, -5.327	1.117, -0.857, -2.052, -5.552	0.125, 2.724, 2.539, 2.764	0.053, 2.415, 2.682, 3.050
P1,P2,P3,P4值	?	>0.05,>0.05, >0.05, <0.01	>0.05,>0.05,<0.05, <0.01	>0.05, <0.05,<0.05, <0.05	>0.05, <0.05,<0.05, <0.05

组别	n	括约肌基础压力	胆总管压力	括约肌幅度	括约肌频率
对照组	10	25.19±7.77	22.35±7.60	8.52±2.27	11.57±2.94
3周组	6	21.25±5.38	18.48±4.94	8.33±3.85	11.50±1.64
6周组	6	27.57±8.67	25.82±8.26	5.26±2.40	7.67±3.44
9周组	6	34.11±11.56	32.15±11.64	5.89±1.41	7.33±3.27
12周组	6	52.38±12.84	50.11±12.59	5.11±2.59	6.60±3.51
t1, t2,t3,t4值	?	1.088, -0.569, -1.857, -5.327	1.117, -0.857, -2.052, -5.552	0.125, 2.724, 2.539, 2.764	0.053, 2.415, 2.682, 3.050
P1,P2,P3,P4值	?	>0.05,>0.05, >0.05, <0.01	>0.05,>0.05,<0.05, <0.01	>0.05, <0.05,<0.05, <0.05	>0.05, <0.05,<0.05, <0.05

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