Relationship of taurine up-regulated gene 1 and miRNA-145 in gastric cancer

doi:10.3877/cma.j.issn.1674-0793.2020.04.011

Abstract

Abstract:

Objective

To detect the expression of taurine up-regulated gene 1 (TUG1) and microRNA-145 (miR-145) in gastric cancer, and to explore their role and relationship in the pathogenesis of gastric cancer.

Methods

From February 2016 to February 2017, 110 cases of gastric cancer underwent radical operation in Hainan Cancer Hospital were selected, the gastric cancer tissues of the patients were taken as the study samples, and the normal tissues beside the cancer were taken as the control samples, the relative expressions of TUG1 and miR-145 in gastric cancer tissue and paracancerous normal tissue were detected by real-time fluorescence quantitative PCR. The relationships between the expressions of TUG1 and miR-145and the clinicopathological data, and the correlation between the relative expressions of TUG1 andmiR-145 and the postoperative survival were analyzed.

Results

Compared with normal tissues, the expression of TUG1 was significantly increased in gastric cancer tissues (P<0.05), the expression of miR-145decreased significantly (P<0.05). There was a negative correlation between the relative expression of TUG1 and miR-145 in gastric cancer (P<0.05). There were significant differences in lymph node metastasis, tumor differentiation and clinical stage between the TUG1 high expression group and the TUG1 low expression group (P<0.05). The expression level of miR-145 was related to lymph node metastasis and clinical stage of patients with gastric cancer (P<0.05). The overall survival rate of gastric cancer patients with TUG1 low expression was significantly higher than that of patients with TUG1 high expression(P=0.010), and the overall survival rate of gastric cancer patients with miR-145 low expression of was significantly lower than that of patients with miR-145 high expression (P<0.001).

Conclusion

TUG1 is significantly highly expressed in gastric cancer, miR-145 is significantly highly expressed in gastric cancer, they are negatively correlated, and have the value of predicting the prognosis of gastric cancer.

Key words: Stomach neoplasms, MicroRNAs, Taurine upregulated gene 1, miRNA-145

Yunhe Hao, Yaoqing Cai, Yiliang Lu. Relationship of taurine up-regulated gene 1 and miRNA-145 in gastric cancer[J]. Chinese Archives of General Surgery(Electronic Edition), 2020, 14(04): 286-289.

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References 15

[1]	Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015[J]. CA Cancer J Clin, 2016, 66(2): 115-132.
[2]	Gao M, Yin H, Fei ZW. Clinical application of microRNA in gastric cancer in Eastern Asian area[J]. World J Gastroenterol, 2013, 19(13): 2019-2027.
[3]	Sant M, Minicozzi P, Primic-Žakelj M, et al. Cancer survival in Europe, 1999-2007: doing better, feeling worse?[J]. Eur J Cancer, 2015, 51(15): 2101-2103.
[4]	Zhou Y, Lu Y, Li R, et al. Prognostic role of long non-coding RNA TUG1 expression in various cancers: A Meta-analysis[J]. Oncotarget, 2017, 8(59): 100499-100507.
[5]	Cui XB, Li S, Li TT, et al. Targeting oncogenic PLCE1 by miR-145 impairs tumor proliferation and matastasis of esophageal squamous cell carcinoma[J]. Oncotarget, 2016, 7(2): 1777-1795.
[6]	郗洪庆，张珂诚，卫勃, 等. 胃癌TNM分期第八版更新在临床诊断治疗中的意义和思考[J]. 中华胃肠外科杂志, 2017, 20(2): 166-170.
[7]	郭波，王文静，赵凌宇, 等. Hsa-miR-106a在胃癌组织中的表达及其临床意义[J]. 山西医科大学学报, 2014, 45(1): 12-15.
[8]	Guan YX, Zhang MZ, Chen XZ, et al. LncRNA SNHG20 participated in proliferation, invasion and migration of breast cancer cells via miR-495[J]. J Cell Biochem, 2018, 119(10): 7971-7981.
[9]	Wang ZQ, He CY, Hu L, et al. Long noncoding RNA UCA1 promotes tumour metastasis by inducing GRK2 degradation in gastric cancer[J]. Cancer Lett, 2017, 408: 10-21.
[10]	Halimi M, Asghari SM, Sariri R, et al. Cellular response to ionizing radiation: A microRNA story[J]. Int J Mol Cell Med, 2012, 1(4): 178-184.
[11]	Berindan-Neagoe I, Monroig Pdel C, Pasculli B, et al. Micro-RNAome genome: A treasure for cancer diagnosis and therapy[J]. CA Cancer J Clin, 2014, 64(5): 311-336.
[12]	Baratieh Z, Khalaj Z, Honardoost MA, et al. Aberrant expression of PlncRNA-1 and TUG1: potential biomarkers for gastric cancer diagnosis and clinically monitoring cancer progression[J]. Biomark Med, 2017, 11(12): 1077-1090.
[13]	窦越，王薇，唐勇. miR-145在胃癌中的表达及其意义[J]. 世界华人消化杂志, 2015, 511(35): 111-115.
[14]	Katsushima K, Natsume A, Ohka F, et al. Targeting the Notch-regulated non-coding RNA TUG1 for glioma treatment[J]. Nat Commun, 2016, 7: 13616.
[15]	申彤，司君利，崔京远, 等. 长链非编码RNA TUG1在胃癌患者中的表达及其对预后的影响[J]. 胃肠病学, 2017, 22(10): 588-591.

临床病理特征		例数	TUG1高表达(80例)	TUG1低表达(30例)	χ2值	P值	miR-145高表达(22例)	miR-145低表达(88例)	χ2值	P值
性别		?	?	?	0.307	0.579	?	?	0.235	0.628
?	男	65	46	19	?	?	12	53	?	?
?	女	45	34	11	?	?	10	35	?	?
年龄(岁)		?	?	?	0.154	0.695	?	?	0.453	0.501
?	>60	48	34	14	?	?	11	37	?	?
?	≤60	62	46	16	?	?	11	51	?	?
肿瘤大小(cm)		?	?	?	1.650	0.199	?	?	2.045	0.153
?	>5	55	43	12	?	?	8	47	?	?
?	≤5	55	37	18	?	?	14	41	?	?
淋巴结转移		?	?	?	8.142	0.004	?	?	5.729	0.017
?	无	60	37	23	?	?	17	43	?	?
?	有	50	43	7	?	?	5	45	?	?
肿瘤分化程度		?	?	?	4.830	0.028	?	?	0.453	0.501
?	低	62	40	22	?	?	11	51	?	?
?	高	48	40	8	?	?	11	37	?	?
TNM分期		?	?	?	13.406	<0.001	?	?	12.462	<0.001
?	Ⅰ~Ⅱ	53	30	23	?	?	18	35	?	?
?	Ⅲ~Ⅳ	57	50	7	?	?	4	53	?	?

临床病理特征		例数	TUG1高表达(80例)	TUG1低表达(30例)	χ2值	P值	miR-145高表达(22例)	miR-145低表达(88例)	χ2值	P值
性别		?	?	?	0.307	0.579	?	?	0.235	0.628
?	男	65	46	19	?	?	12	53	?	?
?	女	45	34	11	?	?	10	35	?	?
年龄(岁)		?	?	?	0.154	0.695	?	?	0.453	0.501
?	>60	48	34	14	?	?	11	37	?	?
?	≤60	62	46	16	?	?	11	51	?	?
肿瘤大小(cm)		?	?	?	1.650	0.199	?	?	2.045	0.153
?	>5	55	43	12	?	?	8	47	?	?
?	≤5	55	37	18	?	?	14	41	?	?
淋巴结转移		?	?	?	8.142	0.004	?	?	5.729	0.017
?	无	60	37	23	?	?	17	43	?	?
?	有	50	43	7	?	?	5	45	?	?
肿瘤分化程度		?	?	?	4.830	0.028	?	?	0.453	0.501
?	低	62	40	22	?	?	11	51	?	?
?	高	48	40	8	?	?	11	37	?	?
TNM分期		?	?	?	13.406	<0.001	?	?	12.462	<0.001
?	Ⅰ~Ⅱ	53	30	23	?	?	18	35	?	?
?	Ⅲ~Ⅳ	57	50	7	?	?	4	53	?	?

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