Expression of lymphatic vessel hyaluronan receptor-1 and vascular endothelial growth factor receptor-3 in gastric cancer

doi:10.3877/cma.j.issn.1674-0793.2009.06.006

Abstract

Abstract:

Objective

To investigate lymphangiogenesis in paritumor and intratumor tissue of gastric cancer by detecting the expression of specific lymphatic endothelium marker of lymphatic vessel hyaluronan receptor-1(LYVE-1) and vascular endothelial growth factor receptor-3(VEGFR-3).

Methods

LYVE-1,VEGFR-3 and their mRNA were examined in 62 gastric cancer tissue by routine immunohistochemical method and real time polymerase chain reaction respectively. Then the density of lymph vessel and the content of LYVE-1 and VEGFR-3 mRNA were calculated.

Results

The density of lymph vessel was higher in paritumor than intratumor tissue.The expression of LYVE-1 and VEGFR-3 protein could not be detected in normal tissue.The density of lymph vessel in paritumor tissue and the mRNA content of LYVE-1 and VEGFR-3 in cancer tissue were correlated with invasion depth,lymphnode metastasis,tumor stage and postoperational recurrence (P<0.05).

Conclusions

LYVE-1 is one kind of more specific lymphatic endothelium marker than VEGFR-3.Lymphangiogenesis can promote the progress and lymph node metastasis in gastric cancer.

Key words: Gastric neoplasm, Hyaluronan receptor, Vascular endothelial growth factor receptor-3, Polymerase chain reaction, Lymphangiogenesis

Xian-jun SUN, Heng MA, Wen-hong HOU, Yan-liang LI, Jie CHAI. Expression of lymphatic vessel hyaluronan receptor-1 and vascular endothelial growth factor receptor-3 in gastric cancer[J]. Chinese Archives of General Surgery(Electronic Edition), 2009, 03(06): 471-475.

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Figures/Tables 8

References 12

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组别	例数	LYVE-1阳性微淋巴管数（LVD）	VEGFR-3阳性脉管数
胃癌组织周边区	62	13.1±1.9	17.8±1.1
胃癌组织中心区	62	7.4±1.2^*	11.4±1.5^*
癌旁正常组织	62	1.7±1.3^*	1.9±1.2^*

组别	例数	LYVE-1阳性微淋巴管数（LVD）	VEGFR-3阳性脉管数
胃癌组织周边区	62	13.1±1.9	17.8±1.1
胃癌组织中心区	62	7.4±1.2^*	11.4±1.5^*
癌旁正常组织	62	1.7±1.3^*	1.9±1.2^*

临床病理因素	例数	P-LVD	P值	I-LVD	P值
年龄（岁）
≤56	23	12.7±1.8	0.154	7.2±1.0	0.259
＞56	39	13.4±1.9		7.5±1.3
性别
男	41	13.3±1.9	0.480	7.2±1.2	0.153
女	21	12.9±1.9		7.7±1.2
浸润深度
T1	7	10.4±0.9	0.000	7.3±1.1	0.863
T2	12	12.6±1.5		7.6±1.4
T3	32	14.0±0.9		7.3±1.3
T4	11	14.7±2.1		7.3±0.8
分化程度
低	20	12.9±2.1	0.416	7.4±1.3	0.846
中	28	13.5±1.8		7.4±1.2
高	14	12.9±1.8		7.6±1.3
淋巴结转移
无	20	10.9±1.1	0.000	7.5±1.0	0.837
有	42	14.2±1.0		7.4±1.3
分期
I	8	10.3±0.7	0.000	7.3±1.2	0.571
II	11	12.1±1.7		7.7±1.3
III	34	13.6±1.0		7.5±1.3
IV	9	14.6±1.8		7.1±1.0
远处转移
无	57	13.1±1.8	0.197	7.4±1.2	0.446
有	5	14.2±2.5		7.0±1.0
随访复发或死亡
无	49	10.5±0.9	0.000	7.2±1.0	0.571
有	13	13.9±1.4		7.4±1.3

临床病理因素	例数	P-LVD	P值	I-LVD	P值
年龄（岁）
≤56	23	12.7±1.8	0.154	7.2±1.0	0.259
＞56	39	13.4±1.9		7.5±1.3
性别
男	41	13.3±1.9	0.480	7.2±1.2	0.153
女	21	12.9±1.9		7.7±1.2
浸润深度
T1	7	10.4±0.9	0.000	7.3±1.1	0.863
T2	12	12.6±1.5		7.6±1.4
T3	32	14.0±0.9		7.3±1.3
T4	11	14.7±2.1		7.3±0.8
分化程度
低	20	12.9±2.1	0.416	7.4±1.3	0.846
中	28	13.5±1.8		7.4±1.2
高	14	12.9±1.8		7.6±1.3
淋巴结转移
无	20	10.9±1.1	0.000	7.5±1.0	0.837
有	42	14.2±1.0		7.4±1.3
分期
I	8	10.3±0.7	0.000	7.3±1.2	0.571
II	11	12.1±1.7		7.7±1.3
III	34	13.6±1.0		7.5±1.3
IV	9	14.6±1.8		7.1±1.0
远处转移
无	57	13.1±1.8	0.197	7.4±1.2	0.446
有	5	14.2±2.5		7.0±1.0
随访复发或死亡
无	49	10.5±0.9	0.000	7.2±1.0	0.571
有	13	13.9±1.4		7.4±1.3

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