To establish animal models of orthotopic transplantation of human gastric carcinoma and provide the visible research method to monitor tumor micrometastases with in-vivo imaging system.

The lentiviral expression vectors carrying luc2, eGFP and puromycin genes was constructed. BGC-823 -eGFP-luc2 stable strain was screened after BGC-823 cell line was infected by the lentivirus. Then animal models with subcutanious implatation and orthotopic transplantation were established.

BGC-823-eGFP-luc2 cell stably expressing green fluorescent protein and firefly luciferase was obtained. The animal models of subcutaneous implantation and orthotopic transplantation were successfully established.

The method of lentiviral infection is suitable for the establishment of human gastric cancer BGC-823-eGFP-luc2 cell strain, which can be used to establish human gastric cancer subcutaneously and orthotopic transplantation models complicated by metastasis rapidly. The in vivo imaging system can be used to monitor the occurrence and development of distal metastasis of gastric cancer.