Expression and clinical significance of Apolipoprotein B, sex hormone binding globulin and tissue inhibitors of metalloproteinase 1 in Budd-Chiari syndrome of inferior vena cava in northern Anhui

doi:10.3877/cma.j.issn.1674-0793.2023.04.003

Abstract

Abstract:

Objective

To investigate the expression and clinical significance of Apolipoprotein B (ApoB), sex hormone binding globulin (SHBG) and tissue inhibitors of metalloproteinase 1 (TIMP-1) in serum of patients with primary inferior vena cava diaphragm type Budd-Chiari syndrome (BCS) in northern Anhui.

Methods

Sixteen patients diagnosed with primary inferior vena cava septal BCS in northern Anhui were selected as the experimental group and eight healthy adults were selected as the control group. The contents of ApoB, SHBG and TIMP-1 in serum of the two groups were detected by enzyme-linked immunosorbent assay (ELISA). Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficiency of differential protein in BCS. qPCR was used to detect the expression abundance of the three protein genes in human umbilical vein endothelial cells (HUVEC).

Results

ELISA results showed that the expression levels of serum ApoB and SHBG were not statistically significant between the two groups (t=0.93, 1.12, P=0.36, 0.27), and the expression level of serum TIMP-1 in the experimental group was lower than that in the control group (t=2.31, P<0.05). ROC curve analysis showed that the area under curve (AUC) of TIMP-1 was 0.75. qPCR indicated that ApoB and SHBG were lowly expressed in HUVEC, while TIMP-1 expression was high.

Conclusion

TIMP-1 may be correlated with primary septal BCS of inferior vena cava in northern Anhui, and can be used as a potential serum biomarker for further study.

Key words: Apolipoprotein B, Sex hormone binding globulin, Tissue inhibitors of metalloproteinase 1, Budd-Chiari syndrome

Longfei Zhang, Yong Gao, Yigang Zhang, Chaowen Yu, Delang Liu, Ya Liu, Shiyuan Chen. Expression and clinical significance of Apolipoprotein B, sex hormone binding globulin and tissue inhibitors of metalloproteinase 1 in Budd-Chiari syndrome of inferior vena cava in northern Anhui[J]. Chinese Archives of General Surgery(Electronic Edition), 2023, 17(04): 252-256.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2023.04.003

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References 21

[1]	Zu M, Xu H, Zhang Q, et al. Review of Budd-Chiari syndrome[J]. J Interv Med, 2020, 3(2): 65-76.
[2]	高涌, 余朝文, 陈世远, 等. 皖北地区布加综合征的流行病学及临床特征分析[J]. 中华血管外科杂志, 2016, 1(3): 164-168.
[3]	刘亚, 高涌, 余朝文, 等. 布加综合征患者血清差异蛋白分析[J]. 中华实验外科杂志, 2020, 37(5): 840-842.
[4]	Valla DC. Budd-Chiari syndrome/hepatic venous outflow tract obstruction[J]. Hepatol Int, 2018, 12(Suppl 1): 168-180.
[5]	Qi X, Yang Z, Bai M, et al. Meta-analysis: the significance of screening for JAK2V617F mutation in Budd-Chiari syndrome and portal venous system thrombosis[J]. Aliment Pharmacol Ther, 2011, 33(10): 1087-1103.
[6]	Singh N, Sharma A, Sazawal S, et al. Prevalence of JAK2V617F mutation in deep venous thrombosis patients and its clinical significance as a thrombophilic risk factor: Indian perspective[J]. Clin Appl Thromb Hemost, 2015, 21(6): 579-583.
[7]	刘春江. 皖北地区布加综合征人群分布及JAK2 V617F、exon12基因点突变检测的研究[D]. 蚌埠: 蚌埠医学院, 2015.
[8]	Sniderman AD, Thanassoulis G, Glavinovic T, et al. Apolipoprotein B particles and cardiovascular disease: A narrative review[J]. JAMA Cardiol, 2019, 4(12): 1287-1295.
[9]	Goldštajn MŠ, Toljan K, Grgic F, et al. Sex hormone binding globulin (SHBG) as a marker of clinical disorders[J]. Coll Antropol, 2016, 40(3): 211-218.
[10]	Li Y, Fang L, Yan Y, et al. Association between human SHBG gene polymorphisms and risk of PCOS: A meta-analysis[J]. Reprod Biomed Online, 2021, 42(1): 227-236.
[11]	Shen J, Song J B, Fan J, et al. Distribution and dynamic changes in matrix metalloproteinase (MMP)-2, MMP-9, and collagen in an in stent restenosis process[J]. Eur J Vasc Endovasc Surg, 2021, 61(4): 648-655.
[12]	荣钰. 血清MMP-9，TIMP-1及VEGF水平检测对冠状动脉支架内再狭窄诊断价值的研究[D]. 呼和浩特: 内蒙古医科大学, 2021.
[13]	Ries C. Cytokine functions of TIMP-1[J]. Cell Mol Life Sci, 2014, 71(4): 659-672.
[14]	Vandooren J, Born B, Solomonov I, et al. Circular trimers of gelatinase B/matrix metalloproteinase-9 constitute a distinct population of functional enzyme molecules differentially regulated by tissue inhibitor of metalloproteinases-1[J]. Biochem J, 2015, 465(2): 259-270.
[15]	邸飞, 李洪利, 赵继宗, 等. 基质金属蛋白酶-9及组织基质金属蛋白酶抑制剂-1在脑动静脉畸形组织中的表达及其与出血的关系[J]. 中国卒中杂志, 2009, 4(7): 576-580.
[16]	何霜霜, 吉沥, 孙燕宁. 无水乙醇介入栓塞治疗对脑动静脉畸形患儿神经功能的影响[J]. 河北医学, 2022, 28(1): 102-107.
[17]	刘传玉, 刘程伟, 袁魁, 等. MMP-9、TIMP-1在曲张大隐静脉中的表达及意义[J]. 黑龙江医药科学, 2014, 37(4): 18-19.
[18]	李蕾, 陈黎明, 赵传艳, 等. 血管内皮生长因子对血管内皮细胞ADAMTS5、ADAMTS4及MMP-9表达的影响[J]. 中国老年学杂志, 2013, 33(19): 4759-4761.
[19]	王佾, 张辉, 郭成浩, 等. 下腔静脉隔膜阻塞型布-加综合征的病理学及病因学研究[J]. 介入放射学杂志, 2008, 17(7): 500-503.
[20]	韩新强, 祖茂衡. 血管性血友病因子、内皮素-1和血管内皮生长因子在下腔静脉隔膜型布-加综合征中异常表达的意义[J]. 中华放射学杂志, 2011, 45(6): 580-583.
[21]	Xu M, Cao L, Zhang X, et al. miR-3133 inhibits proliferation and angiogenesis by targeting the JUNB/VEGF pathway in human umbilical vein endothelial cells[J]. Oncol Rep, 2020, 44(4): 1699-1708.

引物名称	引物序列（5'→3'）	引物长度（bp）
H-Actin-S	CACCCAGCACAATGAAGATCAAGAT	25
H-Actin-A	CCAGTTTTTAAATCCTGAGTCAAGC	27
TIMP-1-F	CTTCTGGCATCCTGTTGTTG	20
TIMP-1-R	AGAAGGCCGTCTGTGGGT	18
SHBG-F	GCCCAGGACAAGAGCCTATC	20
SHBG-R	CCTTAGGGTTGGTATCCCCATAA	23
ApoB-F	CAGCTGATTGAGGTGTCCAG	20
ApoB-R	CACTGGAGGATGTGAGTGGA	20

引物名称	引物序列（5'→3'）	引物长度（bp）
H-Actin-S	CACCCAGCACAATGAAGATCAAGAT	25
H-Actin-A	CCAGTTTTTAAATCCTGAGTCAAGC	27
TIMP-1-F	CTTCTGGCATCCTGTTGTTG	20
TIMP-1-R	AGAAGGCCGTCTGTGGGT	18
SHBG-F	GCCCAGGACAAGAGCCTATC	20
SHBG-R	CCTTAGGGTTGGTATCCCCATAA	23
ApoB-F	CAGCTGATTGAGGTGTCCAG	20
ApoB-R	CACTGGAGGATGTGAGTGGA	20

组别	ApoB(mg/L)	SHBG(nmol/L)	TIMP-1(mg/L)
试验组	0.43±1.18	30.96±53.32	0.27±0.27
对照组	0.03±0.03	9.67±2.92	0.58±0.39
t值	0.93	1.12	2.31
P值	0.36	0.27	0.03

组别	ApoB(mg/L)	SHBG(nmol/L)	TIMP-1(mg/L)
试验组	0.43±1.18	30.96±53.32	0.27±0.27
对照组	0.03±0.03	9.67±2.92	0.58±0.39
t值	0.93	1.12	2.31
P值	0.36	0.27	0.03

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